125µg/mLZ-Lys-Phe-Arg-pNA x 2HCl (Z-KFR-pNA) Merck 233500
in HEPES/NaCl buffer
200 KIU/mL (final) Aprotinin Sigma 10818
Plasma (citrate plasma is good)
Mix plasma with kaolin (any amount is excess) Sigma K7375
Use as 100% standard, make dilution row with untreated plasma
200µl Reagent solution
10µl Plasma or standard
read kinetic over 10min at l = 405nm
The substrate is not in a saturation concentration.
J.M. Grunkemeier, et al., Hemocompatibility of treated polystyrene substrates: Contact activation, platelet adhesion, and procoagulant activity of adherent platelets. J Biomed Mater Res, 1998. 41: 657-70.