450 ml DMEM - High glucose
50 ml Fetal Bovine Serum
0.5 ml Gentamycin (50 mg/ml)
Cells grow as a suspension culture and double approximately every 20-24 hours. Recommended cell density between 0.2 x 106 and 1 x 106 cells/ml. A recommended maintenance schedule is to split the cells 1:4 every other day.
 The AIIB2 and BIIG2 hybridoma lines developed by Caroline H. Damsky was obtained from the Developmental Studies Hybridoma Bank (DSHB) developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242.