Culture Conditions for STRO-1 Cell Line[1]      14.03.2003

Complete Medium (recommended)

450 ml   RPMI 1640                              Biochrom T121

50 ml    Fetal Bovine Serum (heat inactivated has benefit)

5 ml     N-Acetyl L-Alanyl L-Glutamine          Biochrom K0202
         (200mM)

5 ml     Na Pyruvate (10mM – 1.10mg/ml)

0.5 ml   Gentamycin (50 mg/ml)                  Biochrom A271-23

 

additional supplement (own experience):

5 ml     MEM Vitamines (100x)                   Biochrom K0373

10 ml    Amino Acids (50x)                      Biochrom K0363

         Glucose

Maintenance of Cells in Culture

Cells grow as a suspension culture and double approximately every 20-24 hours. Recommended cell density between 0.2 x 106 and 1 x 106 cells/ml. A recommended maintenance schedule is to split the cells 1:4 every other day.

Comments

Cells died rapidly in the original medium without Gentamycin. Heat inactivated Serum, Gentamycin and other supplements to the RPMI medium helped.

Cells grow very well in serum free medium Hybridomed (Biochrom F8055).



[1] The STRO-1 hybridoma developed by Beverly Torok-Storb was obtained from the Developmental Studies Hybridoma Bank (DSHB) developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242.