DAPI-Stain                               04.02.2003

Stock Solution (50µg/ml)    -20°C

500µg    4',6-Diamidine-2'-phenylindole dihydrochloride

                                           (DAPI)      Merck 1.24653

in 10ml Methanol

Staining Solution

24.5ml   Methanol

24.5ml   PBS

1ml      DAPI Stock Solution

Notes

DAPI is used at the final concentration 0.1 to 1µg/ml

It stains AT-rich  single- and double stranded DNA

Required are permeabilized cells, as it does not pass the membranes.

 

Excitation maximum DAPI at l=340nm

Fluorescence maximum DAPI at l=588nm