Hydrogen Peroxide (H2O2) 24.06.03

Reagent

8.9 ml Aqua dest.

100 l TMB diHCl Solution (6 mg/ml in Water) Aldrich 86,151-0
(3,3,5,5-Tetramethylbenzidine dihydrochloride)
1000 l 1 mol/l Potassium Phosphate buffer pH 6.5

10 l Horseradish Peroxidase, 1000U/ml SIGMA P8125

Measurement

200 l Reagent per well
20 l Sample or standard

mix for 3 to 5 min

50 l H2SO4 (1 mol/l)

read at l = 450 nm against blank