0.5ml Paraformaldehyde 37%
400µl fetal calf serum
5µl CaCl2 stock solution 1mol/l
20min Fixation in PBS-Formalin
3x10min wash in PBS
5min 0.2% Triton X/PBS-FBS pH 7.3, 0°C
3x10min wash in PBS-FBS
min 1h primary antibody in PBS-FBS at RT
3x10min wash in PBS-BSA
min 1h sec. antibody 1:50 in PBS-FBS
Phalloidin TRITC 0.1-1µM (Stock 100µM in
2x10min wash in PBS-FBS
Rat Bone Marrow Cells on stainless steel. 8h adhesion time.
 D.L. Spector and H.C. Smith, Redistribution of U-snRNPs during mitosis. Exp Cell Res, 1986. 163: 87-94.