Enzyme activity in the sample: 1 mU/ml up to 1 U/ml
4.92g Tris Base
Ad 500 ml A. dest, pH 7.2 with HCl
297µl NADH 10mg/ml in Buffer Roche 128 023
187µl Pyruvat 10mg/ml in Buffer Fluka 15990
LDH stock solution Roche 107 042
From 1 U/ml always divide by 50% to 2-6U/ml and 0 U/ml
200µl Reagent solution
measure kinetic, E340 every 2 minutes for 20 minutes.
Compare with standards (approx 1 mU/ml bis 1 U/ml)
Double concentration of NADH is used compared with the reference
The reaction is started with the enzyme
For cell culture medium use a 1:10 and 1:50 dilution.
 A. Racher, LDH Assay, in Cell and tissue culture: Laboratory procedures in biotechnology, A. Doyle and J.B. Griffiths, Eds. 1998, John Wiley & Sons: Chichester, New York, Weinheim. p. 71-5.