(after Coomassie-stain and destaining)
50 ml Na2CO3 (30% wAnhydrate/v in Water)
270 µl Formaldehyde 37%
to 500 ml with Aqua a.i.
2x10min Wash with 9.5% ethanol
2x10min Wash with Aqua demin.
30min Incubation with sodium-thiosulfate (0.1%) Sigma
2x10min Wash with Aqua demin
1x10min Wash with Aqua a.i.
30min Incubation in Silver-Nitrate (0.1%) Merck 1512
rinse with Aqua a.i.
ca.10mindevelop (Developer, exchange once)
10min Stop with 1% Acetic Acid
2x5min Wash with Aqua demin.
 J. Heukeshoven and R. Dernick, Simplified method for silverstaining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis, 1985. 6: 103-12.