Peroxidase 11.02.03

Reagent

100 l TMB diHCl Solution (6 mg/ml in Water) Aldrich 86,151-0
(3,3,5,5-Tetramethylbenzidine dihydrochloride)
1 ml 1 mol/l Potassium Phosphate buffer pH 6.5

8.9 ml Aqua dest.

15 l H2O2 30%

Measurement

180 l Reagent per well
20 l Sample

Kinetic measurement at l = 655 nm

or

stop with 50 l H2SO4 (1 mol/l)

read at l = 450 nm