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Methods of Biomaterials Testing

Lecture

Topic

Content

Lecture 1

Introduction

Introduction
Definitions: Biomaterials, Biocompatibility, Biofunctionality
Requirements for “Testing”, Standards, Certified Testing, “experimental” testing, statistics

Lecture 2

Cell culture and tissue engineering

structure of tissue, cells, substructure of cells
Cell culture: definition, possibilities, limitations
primary cells: examples, advantages, limitations
cell lines: dto.
co-culture
tissue engineering
principle handling

Lecture 3

Enzymes

Mass Action Law
Principles of enzymatic reactions
Basics about enzymes
Lambert Beer’s Law
Examples of
- Alkaline Phosphatase and
- Lactate Dehydrogenase (LDH) determination

Lesson 4

Antibodies

Structure of Antibodies
Terminology
Applications:
- Immunochemistry
- indirect Immunochemistry
- Immunofluorescence
- Flow Cytometry
- ELISA
- Immonoblot (Westernblot)

Lesson 5

Molecular Biology

Structure and properties of DNA
DNA in situ hybridization
Polymerase Chain Reaction (PCR)
RNA Transcription
RNA in situ hybridization
reverse transcription PCR (rt-PCR)
realtime PCR
Microarray

Lesson 6

Recent Physical Methods

Atomic Force Microscopy
Quartz Crystal Microbalance (QCM)
Surface Plasmon Resonance (SPR)

Lesson 7

Life and Death

Aspects of Cell Death
Apoptosis vs. Necrosis
Quantification of Necrosis
Analysis of Apoptosis
standardized toxicity tests
vitality and proliferation tests

Lesson 8

Principle biological reactions

Protein adsorption
Vroman effect
Conformation changes
Cell spreading and adherence
Complement system
Heat schock response
Cell differentiation
Cytokines and mediators

Lesson 9

Special Biology: Bone

Differentiation of osteoblasts/ osteocytes
Extracellular Matrix
Osteoclasts
Bone as organ
Animal models

Lesson 10

Special Biology: Blood and vessel wall

Triade of Virchow
Red blood cells
White blood cells
- reactive oxygen Species
Thrombocytes
Protein adsorption
Blood clotting cascade
Blood vessel anatomy
Endothelial cells
- nitric oxide
Smooth muscle cells

Lesson 11

Basics on Pharmcokinetics and Drug release

 

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letzte Aktualisierung: 01.05.2006