Lecture Methods of Biomaterials Testing

Lecture 1

Definitions: Biomaterials, Biocompatibility, Biofunctionality
Requirements for “Testing”, Standards, Certified Testing, “experimental” testing, statistics

Lecture 2

structure of tissue, cells, substructure of cells
Cell culture: definition, possibilities, limitations
primary cells: examples, advantages, limitations
cell lines: dto.
tissue engineering
principle handling

Lecture 3

Mass action law
Principles of enzymatic reactions
Basics about enzymes
Lambert Beer’s Law
Examples of
– Alkaline Phosphatase and
– Lactate Dehydrogenase (LDH) determination

Lecture 4

Structure of Antibodies
– Immunochemistry
– indirect Immunochemistry
– Immunofluorescence
– Flow Cytometry
– Immonoblot (Westernblot)

Lecture 5

Structure and properties of DNA
DNA in situ hybridization
Polymerase Chain Reaction (PCR)
RNA Transcription
RNA in situ hybridization
reverse transcription PCR (rt-PCR)
realtime PCR

Lecture 6

Atomic Force Microscopy
Quartz Crystal Microbalance (QCM)
Surface Plasmon Resonance (SPR)

Lecture 7

Aspects of Cell Death
Apoptosis vs. Necrosis
Quantification of Necrosis
Analysis of Apoptosis
standardized toxicity tests
vitality and proliferation tests

Lecture 8

Protein adsorption
Vroman effect
Conformation changes
Cell spreading and adherence
Complement system
Heat schock response
Cell differentiation
Cytokines and mediators

Lecture 9

Special biology: Bone

Differentiation of osteoblasts/ osteocytes
Extracellular Matrix
Bone as organ
Animal models

Lecture 10

Special biology: Blood and vessel wall

Triade of Virchow
Red blood cells
White blood cells
– reactive oxygen Species
Protein adsorption
Blood clotting cascade
Blood vessel anatomy
Endothelial cells
– nitric oxide
Smooth muscle cells

Lecture 11